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Dual photonic assay successfully reports changes due to changes in CTSL concentration and inhibition of CTSL activity in human serum. Baseline concentration and activity of pooled normal human serum <t>(PNHS)</t> alone, doped with 10 ng·mL –1 CTSL, and doped with 10 ng·mL –1 CTSL plus inhibitor were recorded. Serum doped with 10 ng·mL –1 CTSB produces a response statistically indistinguishable from that produced by serum alone, demonstrating the specificity of the assay. Each value represents the average of 6 replicate measurements, with error bars plus/minus one standard deviation.
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Dual photonic assay successfully reports changes due to changes in CTSL concentration and inhibition of CTSL activity in human serum. Baseline concentration and activity of pooled normal human serum (PNHS) alone, doped with 10 ng·mL –1 CTSL, and doped with 10 ng·mL –1 CTSL plus inhibitor were recorded. Serum doped with 10 ng·mL –1 CTSB produces a response statistically indistinguishable from that produced by serum alone, demonstrating the specificity of the assay. Each value represents the average of 6 replicate measurements, with error bars plus/minus one standard deviation.

Journal: ACS Sensors

Article Title: A Dual-Readout Photonic Sensor for Simultaneous Measurement of Enzyme Activity and Concentration

doi: 10.1021/acssensors.5c01760

Figure Lengend Snippet: Dual photonic assay successfully reports changes due to changes in CTSL concentration and inhibition of CTSL activity in human serum. Baseline concentration and activity of pooled normal human serum (PNHS) alone, doped with 10 ng·mL –1 CTSL, and doped with 10 ng·mL –1 CTSL plus inhibitor were recorded. Serum doped with 10 ng·mL –1 CTSB produces a response statistically indistinguishable from that produced by serum alone, demonstrating the specificity of the assay. Each value represents the average of 6 replicate measurements, with error bars plus/minus one standard deviation.

Article Snippet: Pooled Normal Human Serum (PNHS) was purchased from Innovative Research (Novi, MI).

Techniques: Concentration Assay, Inhibition, Activity Assay, Produced, Standard Deviation